Every year, an estimated 48 million Americans get sick from foodborne illnesses, resulting in some 128,000 hospitalizations and 3,000 deaths, according to the U.S. Centers for Disease Control and Prevention. This public health problem is compounded by billions in economic damage from product recalls, highlighting the need to rapidly and accurately determine the sources of foodborne illnesses.With the increasing complexity of global supply chains for the myriad foods available to consumers, however, the task of tracing the exact origin of contaminated items can be difficult.In a novel solution that can help determine the origin of agricultural products and other goods, Harvard Medical School (HMS) scientists have developed a DNA-barcoded microbial system that can be used to label objects in an inexpensive, scalable and reliable manner.A flask of DNA-barcoded spores. Photos courtesy of Jason QianReporting in Science on June 4, the research team describes how synthetic microbial spores can be safely introduced onto objects and surfaces at a point of origin, such as a field or manufacturing plant, and be detected and identified months later.The spores are derived from baker’s yeast and a common bacterial strain used in a wide variety of applications, such as probiotic dietary supplements, and designed to be incapable of growing in the wild to prevent adverse ecological effects.“Spores are in many ways an old-school solution and have been safely sprayed onto agricultural goods as soil inoculants or biological pesticides for decades. We just added a small DNA sequence we can amplify and detect,” said study corresponding author Michael Springer, associate professor of systems biology in the Blavatnik Institute at HMS.“We also worked hard to make sure this system is safe, using commonplace microbial strains and building in multiple levels of control,” Springer added. “We hope it can be used to help solve problems that have enormous public health and economic implications.”In recent years, scientists have learned a great deal about the interactions between microbes and their environments. Studies show that microbial communities in homes, on cell phones, on human bodies and more have unique compositions, similar to fingerprints. Attempts to use microbial fingerprints to identify provenance can be time consuming and are not easily scaled, however.The use of custom-synthesized DNA sequences as barcodes has been shown in principle to be effective for labeling food and other items. To be widely useful, DNA barcodes must be produced cheaply in large volumes, persist on objects in highly variable environments, and able to be reliably and rapidly decoded — hurdles that have thus far not been overcome because DNA is fragile.Heavy-duty packagingIn their study, Springer and colleagues set out to determine if DNA barcodes packaged within microbial spores, which can be sprayed onto crops and identified months later, could help solve these challenges.Many microorganisms, including bacteria, yeasts and algae, form spores in response to harsh environmental conditions. Analogous to seeds, spores allow microorganisms to remain dormant for extraordinarily long periods and survive extreme conditions such as high temperatures, drought and UV radiation.The research team created custom-made DNA sequences that they integrated into the genomes of the spores of two microorganisms — Saccharomyces cerevisiae, also known as baker’s yeast, and Bacillus subtilis, a common bacterium that has numerous commercial uses, including as a dietary probiotic, a soil inoculant and a fermenting agent in certain foods. These spores can be cheaply grown in the lab in large numbers.The synthetic DNA sequences are short and do not code for any protein product and are thus biologically inert. Inserted into the genome in tandem, the sequences are designed so that billions of unique barcodes can be created.The team also ensured that DNA-barcoded spores could not multiply, grow and spread in the wild. They did so by using microbial strains that require specific nutritional supplementation and by deleting genes required for the spores to germinate and grow. Experiments involving from hundreds of millions to more than a trillion of the modified spores confirmed that they are unable to form colonies.To read the DNA barcodes, the researchers used an inexpensive CRISPR-based tool that can detect the presence of a genetic target rapidly and with high sensitivity. The technology, called SHERLOCK, was developed at the Broad Institute of MIT and Harvard, in a collaboration led by institute members James Collins and Feng Zhang.“Spores can survive in the wild for an extremely long time and are a great medium for us to incorporate DNA barcodes into,” said study co-first author Jason Qian, a graduate student in systems biology at HMS. “Identifying the barcodes is straightforward, using a blue light source, an orange plastic filter and a cell phone camera. We don’t envision any challenges for field deployability.” Real worldThe team examined the efficacy of their barcoded microbial spore system through a variety of experiments.They grew plants in the laboratory and sprayed each plant with different barcoded spores. A week after inoculation, a leaf and a soil sample from each pot were harvested. The spores were readily detected, and even when the leaves were mixed together, the team could identify which pot each leaf came from.When sprayed onto grass outside and exposed to natural weather for several months, spores remained detectable, with minimal spread outside the inoculated region. On environments such as sand, soil, carpet and wood, the spores survived for months with no loss over time, and they were identified after disturbances such as vacuuming, sweeping and simulated wind and rain.Researchers built a sandpit to test the barcoded microbial spore system in simulated real-world environments.Spores are very likely to persist through the conditions of a real-world supply chain, according to the researchers. As a proof-of-principle, they tested dozens of store-bought produce items for the presence of spores of Bacillus thuringiensis (Bt), a bacterial species that is widely used as a pesticide. They correctly identified all Bt-positive and Bt-negative plants.In additional experiments, the team built a 100-square-meter (~1,000 square feet) indoor sandpit and found that the spread of spores was minimal after months of simulated wind, rain and physical disturbances.They also confirmed that spores can be transferred onto objects from the environment. Spores were readily identified on the shoes of people who walked through the sandpit, even after walking for several hours on surfaces that were never exposed to the spores. However, the spores could not be detected on these surfaces, suggesting that objects retain the spores without significant spread.This characteristic, the team noted, could allow spores to be used to determine whether an object has passed through an inoculated area. They tested this by dividing the sandpit into grids, each labeled with up to four different barcoded spores. Individuals and a remote-control car then navigated the sandpit.They found that they could identify the specific grids that the objects passed through with minimal false positives or negatives, suggesting a possible application as a complementary tool for forensics or law enforcement.The team also considered potential privacy implications, noting that existing technologies such as UV dyes, cell phone tracking and facial recognition are already widely used but remain controversial.“As scientists, our charge is to solve scientific challenges, but at the same time we want to make sure that we acknowledge broader societal implications,” Springer said. “We believe the barcoded spores are best suited for farming and industrial applications and would be ineffective for human surveillance.”Regardless, the use and adoption of this technology should be done with a consideration of ethics and privacy concerns, the study authors said.The researchers are now exploring ways to improve the system, including engineering potential kill-switch mechanisms into the spores, finding ways to limit propagation and examining if the spores can be used to provide temporal information about location history.“Outbreaks of harmful foodborne pathogens such as listeria, salmonella and E. coli occur naturally and frequently,” Springer said. “Simple, safe synthetic biology tools and knowledge of basic biology allow us to create things that have a lot of potential in solving real world safety issues.”Study co-first authors include Zhi-xiang Lu, Christopher Mancuso, Han-Ying Jhuang,Rocío del Carmen Barajas-Ornelas, and Sarah Boswell.Additional authors include Fernando Ramírez-Guadiana, Victoria Jones, Akhila Sonti, Kole Sedlack, Lior Artzi, Giyoung Jung, Mohammad Arammash, Mary Pettit, Michael Melfi, Lorena Lyon, Siân Owen, Michael Baym, Ahmad Khalil, Pamela Silver, and David Rudner.This work was supported by DARPA Biological Robustness in Complex Settings (grant HR001117S0029).
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Dreaming of living as a professional musician by making money in the industry depends on several factors. One of these factor is having a loyal fan base who can take you from just being a mainstream artists to a more professional musician who makes money from his work.Why is a loyal fan base necessary?A large fan base is necessary at some point for an artist to gain fame quick, but it is a loyal fan base that determines how long you will still in the industry. And the success you will accumulate.A person who is not a loyal fan can purchase your work and lose appetite for it quickly, making that artist to be forgotten soon.“Think about how many artists have been ‘the next big thing’ only to be forgotten soon after,” said Tom Hess, a touring guitarist, recording artist and a former member of the band Rhapsody of Fire from 2010 until 2013.However, loyal fans will have value for your product including buying your CD, merchandise and attending all of every concert because they need your autograph frequently as possible.While it is true that CDs and ITunes sale has drop drastically in the music industry around the world and streaming services is taking the music, making music almost free and giving little compensation to artists, a loyal fan base solve the problem of not making money from your music.For international artists like Beyoncé, Abele and Taylor Swift they continue to make millions of dollars through the sale of CDs and downloads despite the decline in CDs and download sales.But these women power to attain such a status is accumulated through their loyal fan base, who are willing to support them in everything.“If you want to make money with your music, a smaller number of dedicated fans are more valuable to you than large number of casualfans,” said Tom Hess.Also, the author of wired magazine Kevin Kelly said: “1,000 loyal fans are enough for an artist to earn a comfortable living. Let’s say you have 1,000 real fans, each spending U$100 per year on your music and merchandise that would equal U$100 per year of income.”How to Grab Loyal FansAs veteran Liberian music critic Ernest Bruce put it fans are the real investors in the music industry, and artists have the service to give. So to keep in direct contact with people who begin supporting your career at the early stage is essential to convert them to loyal fans.Making these newly converted fans feel that they are part of the artist career development, it makes them grow the trust and loyalty over time toward that artist’s career.The direct contact can be archived via social media, autographs signing and free exclusive items. And when this is done, a fan never regrets to support all your work, talking about your music to colleagues and defending you in the midst of criticism.Tom Hess added: “So rather than aiming for becoming an overnight sensation with just one single, aim at creating just 1,000 true fans.This is a realistic goal to achieve, and it will help you make money with your music while maintain a stable music career. You can always grow and expanded from there.”To learn more about how to build a loyal fan base read one of our previous articles: How to Build a Fan Base: Strategy for Liberianartist.Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window)